Identification of new chalcone synthase genes for flower pigmentation in the Japanese and common morning glories.
نویسندگان
چکیده
New cDNA sequences for chalcone synthase (CHS), a key enzyme in the flavonoid biosynthesis, were obtained from the Japanese and common morning glories; they are more closely related to other CHS sequences than the six previously described CHS genes from the same plants. The newly isolated CHS-D gene is abundantly expressed in the pigmented flower buds, while its expression is drastically reduced in the white flower buds. Thus CHS-D appears to produce major CHS transcripts for flower pigmentation.
منابع مشابه
Likelihood Analysis of the Chalcone Synthase Genes Suggests the Role of Positive Selection in Morning Glorie (Ipomoea)
Chalcone synthase (CHS) is a key enzyme in the biosynthesis of flavonoides, which are important for the pigmentation of flowers and act as attractants to pollinators. Genes encoding CHS constitute a multigene family in which the copy number varies among plant species and functional divergence appears to have occurred repeatedly. In morning glories (Ipomoea), five functional CHS genes (A–E) have...
متن کاملFlavonoid genes in petunia: addition of a limited number of gene copies may lead to a suppression of gene expression.
To evaluate the effect of increased expression of genes involved in flower pigmentation, additional dihydroflavonol-4-reductase (DFR) or chalcone synthase (CHS) genes were transferred to petunia. In most transformants, the increased expression had no measurable effect on floral pigmentation. Surprisingly, however, in up to 25% of the transformants, a reduced floral pigmentation, accompanied by ...
متن کاملAnthocyanin Biosynthetic Genes and Their Application to Flower Color Modification through Sense Suppression
zThe coding sequence is compared for each pair of genes. Comparisons were done using the gap program of the Wisconsin GCG sequence analysis package. yCHS = chalcone synthase; CHI = chalcone isomerase; DFR = dihydroflavonol reductase. xFirst row for each plant pair presents the percent amino acid similarity. wSecond row for each plant pair presents the percent amino acid identity. vThird row for...
متن کاملIsolation and characterization of GtMYBP3 and GtMYBP4, orthologues of R2R3-MYB transcription factors that regulate early flavonoid biosynthesis, in gentian flowers
Flavonoids are one of the major plant pigments for flower colour. Not only coloured anthocyanins, but also co-pigment flavones or flavonols, accumulate in flowers. To study the regulation of early flavonoid biosynthesis, two R2R3-MYB transcription factors, GtMYBP3 and GtMYBP4, were identified from the petals of Japanese gentian (Gentiana triflora). Phylogenetic analysis showed that these two pr...
متن کاملDuplication and adaptive evolution of the chalcone synthase genes of Dendranthema (Asteraceae).
Chalcone synthase (CHS) is a key enzyme in the biosynthesis of flavonoids, which are important for the pigmentation of flowers and act as attractants to the pollinators. Genes encoding CHS constitute a multigene family in which the copy number varies among plant species and functional divergence appears to have occurred repeatedly. Plants of the Dendranthema genus have white, yellow, and pink f...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Plant & cell physiology
دوره 38 6 شماره
صفحات -
تاریخ انتشار 1997